研究成果 -- 中国科学院实验海洋生物学重点实验室

研究成果

课题组在海水鱼类及文昌鱼、斑马鱼的早期发育、性别决定与性腺分化、肌肉发育、耐低温响应、快速育种技术等方面取得进展。建立了细胞、个体等水平的海水鱼类基因功能验证平台；初步解析了斑马鱼和文昌鱼胚胎发育过程原肠化运动的关键因子的作用机理；获得了牙鲆和大菱鲆受精卵从胚盘形成到4-细胞期中心体、纺锤体及核的变化图象；初步阐释了牙鲆雌雄表型形成的机制及外源因子对其影响机制；解析了鱼类骨骼肌细胞的发生、发育分化的分子调控机制；通过文昌鱼与高等动物的比较分析，初步阐释了肌细胞发育调控因子的进化特点；利用mRNA和miRNA转录组、代谢组多组学研究，以及生理生化分析，揭示了牙鲆、黄姑鱼等重要海水养殖鱼类对温度胁迫的响应机制及其分子调控机理，获得了耐温相关分子标记；提升了染色体操作早期卵裂抑制技术，诱导并建立了同质雌核发育牙鲆子三代、大菱鲆子二代群体，进一步优化了鲆鲽鱼类三倍体、四倍体诱导技术，显著提高了诱导率；首次报道半滑舌鳎病毒性疾病的暴发；确定鳗弧菌MHK3株六型分泌系统（T6SS）的致病性功能和大西洋鲑养殖主要病原；研制了具有良好效果的多个鱼用疫苗制品。

本研究组先后发表SCI论文130余篇；主编和参与撰写专著10部。获授权国家发明专利25项、国家实用新型专利2项；登记计算机软件著作权1项。研究成果获国家、省部市级学术奖励14项。先后培养博士后9名、研究生近70人，毕业生20余人次赴国外深造，其他人大多活跃在我国海洋生物学相关的科研院所。

代表性论文：

1. Jiao, S., Nie, M., Song, H., Xu, D.*, You, F.* (2020). Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics. Science of The Total Environment, 715, 136940.

2. Wu, Z., Wang, L., Wu, Q., Lu, Y., Song, Z., Li, J., You, F.* (2020). Study on artificial induction, growth and gamete quality of mitogynogenetic turbot Scophthalmus maximus. Aquaculture, 515, 734585.

3. Zou, C., Wang, L., Zou, Y., Wu, Z., Wang, W., Liang, S., Wang, L., You, F.* (2020). Characteristics and sex dimorphism of 17β-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus. Journal of Steroid Biochemistry and Molecular Biology, 199, 105597.

4. Wang, L., Wu, Z., Wang, Y., Liu, M., Song, A., Liu, H., You, F.* (2020). Genetic assessment of a black rockfish, Sebastes schlegelii, stock enhancement program in Lidao Bay, China based on mitochondrial and nuclear DNA analysis. Frontiers in Marine Science, 7, 94.

5. Zou, Y., Peng, L., Weng, S., Liang, D., Fan, Z., Wu, Z., Tan, X., Jiao, S., You, F.* (2019). Characterization and expression of androgen receptors in olive flounder. Gene, 683:184-194.

6. Liu, H., Wu, Z., Zhu, X., Song, Z., Hu, J., Wang, L., Li, J., You, F.* (2018). Comparative performance of growth, vertebral structure and muscle composition in diploid and triploid Paralichthys olivaceus. Journal of Fish Disease, 41:1495-1504.

7. Liang, D., Fan, Z., Zou, Y., Tan, X., Wu, Z., Jiao, S., Li, J., Zhang, P., You, F.* (2018). Characteristics of cyp11a during gonad differentiation of the olive flounder Paralichthys olivaceus. International Journal of Molecular Sciences, 19(9), 2641.

8. Lu, Y., Nie, M., Wang, L., Xiong, Y., Wang, F., Wang, L., Xiao, P., Wu, Z., You, F.* (2018). Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged. Aquaculture, 484, 205-213.

9. Fan, Z., Zou, Y., Jiao, S., Tan, X., Wu Z., Liang, D., Zhang, P., You, F.* (2017). Significant association of cyp19a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus. Comparative Biochemistry and Physiology, Part A, 2017, 208: 70–79.

10. Zheng, Y.^#, Peng, L. M.^#, You, F.*, Zou, Y. X., Zhang, P. J., Chen, S. L. (2015). Establishment and characterization of a fish‐cell line from the brain of Japanese flounder Paralichthys olivaceus. Journal of Fish Biology, 87(1), 115-122.

11. Xiao, P., Jiang, M., Liu, Y., Sun, M., Zhang, L., Li, J., Li, G., Mo, Z.* (2015). Splenic necrosis signs and pathogen detection in cultured half-smooth tongue sole, Cynoglossus semilaevis Günther. Journal of Fish Diseases, 38(1): 103-106.

12. Fan, Z., You, F.*, Wang, L., Weng, S., Wu, Z., Hu, J., Zou, Y., Tan, X., Zhang, P. (2014). Gonadal transcriptome analysis of male and female olive flounder (Paralichthys olivaceus). BioMed Research International, 2014, 291067.

13. Wen, A., You, F.*, Sun, P., Li, J., Xu, D., Wu, Z., Ma, D., Zhang, P. (2014). CpG methylation of dmrt1 and cyp19a promoters in relation to their sexual dimorphic expression in the Japanese flounder Paralichthys olivaceus. Journal of Fish Biology, 84(1), 193-205.

14. Wang, X., Tan, X.*, Zhang P., Zhang, Y., Xu, P. (2014). Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus). J Biosci. 39(5), 849-858.

15. You, F., Xu, J., Zhu, X., Xu, Y., Zhang, P.* (2008). Effect of UV irradiation on sperm of the left-eyed flounder, Paralichthys olivaceus. Journal of the World Aquaculture Society, 39(3), 414-422.

16. Tan, X.^#, Rotllant, J., Li, H., De Deyne, P., Du, S. J.^#,*(2006). SmyD1, a histone methyltransferase, is required for myofibril organization and muscle contraction in zebrafish embryos. Proceedings of the National Academy of Sciences, 103(8), 2713-2718.

代表性授权国家发明专利:

1. 尤锋，张培军，王新成，许建和. 一种牙鲆受精卵第一次卵裂抑制的方法. 国家发明专利，专利号：ZL 2004 1 0050312.X；授权公告日：2008.04.23.

2. 孙鹏，尤锋，杨奔，张培军，徐永立. 一种牙鲆仔稚幼鱼性激素水平测定的样品制备方法. 国家发明专利，专利号：ZL 2007 1 0015287.5；授权公告日：2010.06.23.

3. 吴志昊，尤锋，宋宗诚，王丽娟，范兆飞，曹元水. 一种大菱鲆同质雌核发育鱼苗的诱导方法. 国家发明专利，授权号：ZL2015 1 0069960.8；授权公告日：2017.02.22.

4. 尤锋，吴志昊，胡金伟，马得友，谭训刚，张培军. 一种大菱鲆四倍体鱼苗的批量诱导方法. 国家发明专利，授权号：ZL 2013 1 0039710.0；授权公告日：2015.09.23.

5. 焦爽，范兆飞，尤锋，徐冬冬，谭训刚. 一种牙鲆幼鱼性腺mRNA原位杂交的方法. 国家发明专利，授权号：ZL 2016 1 0080377.1；授权公告日：2019.1.8.

6. 谭训刚，李美洁，王倩，尤锋，焦爽. 牙鲆原始生殖细胞nanos3基因的调控序列及其应用. 国家发明专利，授权号：ZL 2014 1 0336602.4；授权公告日：2017.1.18.

7. 尤锋，郑媛，彭丽敏，邹玉霞，吴志昊，谭训刚，焦爽. 一种牙鲆脑细胞系的构建方法. 国家发明专利，授权号：ZL 2014 1 0071475.X；授权公告日：2015.07.22.

8. 彭丽敏，郑媛，尤锋，吴志昊，邹玉霞. 一种牙鲆精巢细胞系的构建方法. 国家发明专利，授权号：ZL2015 1 0061319.X，授权公告日：2017.11.10.

9. 谭训刚，隋玉嫘，焦爽，吴志昊，李美洁，邹玉霞，梁冬冬，尤锋. 一种牙鲆原始生殖细胞dead end基因及其的应用. 国家发明专利，授权号：ZL2016 1 0079181.0；授权公告日：2019.2.4.

10. 尤锋，吴志昊，王伟，徐冬冬，张毅，张培军，徐永立. 一种提取单个鱼卵仔鱼微量总DNA的简便快速方法. 国家发明专利，授权号：ZL 2011 1 0027643.1；授权公告日：2013.10.23.

海洋鱼类遗传发育及进化