研究成果 -- 中国科学院实验海洋生物学重点实验室

研究成果

课题组在对虾基因组和重要经济性状分子机制解析、遗传操作、免疫调控、新品种培育等基础研究、技术平台创新和产业应用等方面获得了一系列创新性成果。利用多组学技术解析海洋无脊椎动物重要生命现象，在国际上首先完成了凡纳滨对虾和仿刺参的全基因组图谱构建，特别是阐明了对虾基因组中高比例重复序列的结构特点，以及适应底栖生活和频繁蜕皮的遗传学基础；开展了对虾等甲壳动物免疫、性别、生长等重要经济性状的基础生物学前沿研究，揭示了甲壳动物体液免疫和免疫稳态的重要调控途径，鉴定了虾类中多种抗菌肽分子，为对虾抗病药物及制剂研发奠定了重要基础；获取了对虾性别DNA探针，鉴定了多个调控对虾雄性性别分化和性别发育的重要基因，初步揭示了对虾雄性性别分化的分子调控途径；进行了虾类等经济甲壳动物分子育种平台构建和育种实践，开发了多个抗WSSV、副溶血弧菌等对虾中主要病原的分子标记，建立了对虾重要经济性状分子育种技术平台和基因编辑等遗传操作技术平台，加速了对虾优良品种选育。在Nature Communications、PLoS Biology、Molecular Ecology、Frontiers in Immunology、Chemosphere、Food Chemistry等国际重要学术期刊发表研究论文300余篇，授权国际PCT专利2项、国家发明专利20余项；先后培育出凡纳滨对虾国审新品种“科海1号”、“广泰1号”和脊尾白虾国审新品种“科苏红1号”，发挥了重要的产业带动作用；获得省、部级奖励10余项。

代表性论文：

1. Xiaojun Zhang^#, Jianbo Yuan^#, Yamin Sun^#, Shihao Li^#, Yi Gao^#, Yang Yu^#, Chengzhang Liu, Quanchao Wang, Xinjia Lv, Xiaoxi Zhang, Ka Yan Ma, Xiaobo Wang, Wenchao Lin, Long Wang, Xueli Zhu, Chengsong Zhang, Jiquan Zhang, Songjun Jin, Kuijie Yu, Jie Kong, Peng Xu, Jack Chen, Hongbin Zhang, Patrick Sorgeloos, Amir Sagi, Acacia Alcivar-Warren, Zhanjiang Liu, Lei Wang, Jue Ruan, Ka Hou Chu*, Bin Liu*, Fuhua Li* & Jianhai Xiang*. 2019. Penaeid shrimp genome provides insights into benthic adaptation and frequent molting. Nature Communications (2019) 10:356.

2. Xiaojun Zhang^#, Lina Sun^#, Jianbo Yuan^#, Yamin Sun^#, Yi Gao^#, Libin Zhang^#, Shihao Li^#, Hui Dai, Jean-Francois Hamel, Chengzhang Liu,Yang Yu, Shilin Liu, Wenchao Lin, Kaimin Guo, Songjun Jin, Peng Xu, Kenneth B. Storey, Pin Huan, Tao Zhang, Yi Zhou, Jiquan Zhang, Chenggang Lin,Xiaoni Li, Lili Xing, Da Huo, Mingzhe Sun, Lei Wang, Annie Mercier*, Fuhua Li*, Hongsheng Yang*, Jianhai Xiang*. 2017. The sea cucumber genome provides insights into morphological evolution and visceral regeneration. Plos Biology, 15(10): e2003790.

3. Jianbo Yuan, Xiaojun Zhang*, Yi Gao, Xiaoxi Zhang, Chengzhang Liu, Jianhai Xiang, and Fuhua Li*. Adaptation and molecular evidence for convergence in decapod crustaceans to deep-sea hydrothermal vent environments. Molecular Ecology, 2020, 29:3954–3969.

4. Fei Liu^#, Shihao Li^#, Yang Yu, Mingzhe Sun, Jianhai Xiang, Fuhua Li*, 2020. Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei. Chemosphere 239, 124759.

5. Shihao Li, Xinjia Lv, Fuhua Li*, Jianhai Xiang, 2019. Characterization of a lymphoid organ specific anti-lipopolysaccharide factor from shrimp reveals structure-activity relationship of the LPS-binding domains. Frontiers in Immunology 10: 872.

6. Zhang Q^#, Yu Y^#, Wang Q, Liu F, Luo Z, Zhang C, Zhang X, Huang H, Xiang J and Li F*. 2019. Identification of Single Nucleotide Polymorphisms Related to the Resistance Against Acute Hepatopancreatic Necrosis Disease in the Pacific White Shrimp Litopenaeus vannamei by Target Sequencing Approach. Front. Genet. 10:700.

7. Yang Yu^#, Quanchao Wang^#, Qian Zhang, Zheng Luo, Yue Wang, Xiaojun Zhang, Hao Huang, Jianhai Xiang, Fuhua Li*. 2019. Genome Scan for Genomic Regions and Genes Associated with Growth Trait in Pacific White Shrimp Litopeneaus vannamei. Marine Biotechnology 21:374-383.

8. Chengsong Zhang, Fang Su, Shihao Li, Yang Yu, Jianhai Xiang, Jianguo Liu*, Fuhua Li*. 2018. Isolation and identification of the main carotenoid pigment from a new variety of the ridgetail white prawn Exopalaemon carinicauda. Food Chemistry 269, 450–454.

9. Qing Guo, Shihao Li*, Xinjia Lv, Jianhai Xiang, Amir Sagi, Rivka Manor, Fuhua Li*. 2018. A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp. Endocrinology. 159(5):2173–2185.

10. Shihao Li^#, Zhiwei Wang^#, Fuhua Li*, Kuijie Yu and Jianhai Xiang. 2017. A Novel Vascular Endothelial Growth Factor Receptor Participates in White Spot Syndrome Virus Infection in Litopenaeus vannamei. Front. Immunol. 8:1457.

11. Hui Yang, Shihao Li, Fuhua Li* and Jianhai Xiang, 2016. Structure and Bioactivity of a Modified Peptide Derived from the LPS-Binding Domain of an Anti-Lipopolysaccharide Factor (ALF) of Shrimp. Marine Drugs, 14, 96.

12. Fuhua Li, Jianhai Xiang, 2013. Recent advances in researches on the innate immunity of shrimp in China. Developmental and Comparative Immunology 39(1-2): 11-26.

13. Fuhua Li, Jianhai Xiang, 2013. Signaling pathways regulating innate immune responses in shrimp. Fish and Shellfish Immunology 34:973-980.

14. Chengsong Zhang, Fuhua Li, Jianhai Xiang, 2014. Acute effects of cadmium and copper on survival, oxygen consumption, ammonia-N excretion, and metal accumulation in juvenile Exopalaemon carinicauda, Ecotoxicology and Environmental Safety, 104:209-214.

15. Hao Jiang, Fuhua Li, Jiquan Zhang, Jinkang Zhang, Bingxin Huang, Yang Yu, Jianhai Xiang*, 2014. Comparison of protein expression profiles of the hepatopancreas in Fenneropenaeus chinensis with heat-inactivated Vibrio anguillarum and white spot syndrome virus. Marine Biotechnology 16(1)：111-123.

16. Shihao Li, Xiaojun Zhang, Zheng Sun, Fuhua Li*, Jianhai Xiang, 2013. Transcriptome analysis on Chinese shrimp Fenneropenaeus chinensis during WSSV acute infection. PLoS ONE 8(3): e58627.

17. Rong Wen, Fuhua Li*, Shihao Li, Jianhai Xiang, 2014. Function of Shrimp STAT during WSSV Infection. Fish& Shellfish Immunology 38: 354-260.

18. Yumiao Sun, Fuhua Li*, Zheng Sun, Xiaojun Zhang, Shihao Li, Chengsong Zhang, Jianhai Xiang, 2014. Transcriptome analysis of the initial stage of acute WSSV infection caused by temperature change. PLoS ONE 9(3): e90732.

19. Shihao Li, Shuyue Guo, Fuhua Li*, Jianhai Xiang, 2014. Characterization and function analysis of an anti-lipopolysaccharide factor (ALF) from the Chinese shrimp Fenneropenaeus chinensis. Developmental and Comparative Immunology 46: 349-355.

20. Rong Wen, Fuhua Li*, Yusu Xie, Shihao Li, Jianhai Xiang, 2012. A Homolog of Cell Apoptosis Susceptibility Gene Involved in the Ovary Development of Chinese Shrimp Fenneropenaeus chinensis. Biology of Reproduction 86(1):1-7.

海洋甲壳动物生殖发育与遗传工程